Human GABRA1 and GABRB3 were cloned into pEG BacMam plasmids, respectively; a plasmid expressing human GABRG2 from the CMV promoter and EGFP from the EF1a promoter was constructed to ensure the ...

Embryonic polarity of invertebrates, amphibians and fish is specified largely by maternal determinants, which fixes cell fates early in development. In contrast, amniote embryos remain plastic and can ...