Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular and molecular basis governing microglia maintenance ...

进一步共定位分析发现GP5促进了内质网与线粒体接触（图2F-G）。 图2 PRRSV GP5负责内质网-线粒体的接触。用EGFP、GP5-EGFP或NSP2-EGFP质粒转染(A) MARC-145细胞24 h。采用免疫荧光法检测线粒体（Tom20）的形态。(B)从(A)中获得的线粒体网络数量的定量分析（n = 30）.(C)(A ...

P<0.01. 12 propranolol treated, 12 metoprolol treated, and 13 vehicle brains were analyzed. (C through K) Representative light sheet microscopy scanning pictures of brains from ccm2 CRISPR adult ...

Microscopic control of cells was performed using the fluorescence microscope EVOS FL Cell Imaging System. EGFP was detected in the GFP channel, and Katushka2S, in the RFP channel. In the images of ...

2000; Tamamaki et al., 2003). The most commonly used tag is the green fluorescent protein (EGFP), as there is a wide availability of vectors and of transgenic mice leading to cell specific EGFP ...