在成功开发出用于病毒重建的自辅助病毒启动方法后，作者随后建立了一个合成基因组学的基因组组装过程来组装非洲猪瘟病毒基因组，这类似于作者先前报道的用于人类疱疹病毒的方法。作者通过酵母中的转化相关重组（TAR）技术，从 12 个重叠的野生型或修饰片段（或 ...

Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular and molecular basis governing microglia maintenance ...

Edited by Joseph Kieber, The University of North Carolina at Chapel Hill, Chapel Hill, NC; received November 27, 2024; accepted January 7, 2025 ...

Clonal analysis of murine hematopoiesis. A: Experimental outline for lineage tracing of unperturbed hematopoiesis. M2/HSB/Tn mice were labeled with Dox for four weeks. The DsRed + fractions of the ...

DsRed, a recently cloned red fluorescent protein, has attracted great interest as an expression tracer and fusion partner for multicolor imaging. We report that three-photon excitation (λ <760 nm ...