Ltd. Escherichia coli DH5α competent cells were purchased from Vazyme Biotech (Nanjing) Co., Ltd. PGEX-6P-1-CSFV NS5A plasmids, human embryonic kidney 293T cells (HEK-293T), pCDH-CMV-MCS-EF1 ...

The GST-tagged FRMD8 expression plasmid was generated by inserting PCR-amplified fragments into pGEX-4T-1 vector. All constructs were confirmed by DNA sequencing. For transient transfection, cells at ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Lipid transfer proteins mediate the ...

CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China University of Chinese Academy of Sciences, Beijing ...

研究人员在研究过程中，主要运用了以下关键技术方法：首先是重组细菌的构建与表达，利用大肠杆菌 TOP10 作为宿主菌株，PGEX-2T 作为质粒载体，构建表达载体来表达 Hv1a；其次是蛋白的提取与纯化，通过特定的裂解缓冲液裂解细菌细胞，再经离心、GST 亲和层析 ...