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10 天
噬菌体 Eryne 的基因组解析:新型大肠杆菌噬菌体的探索之旅
具体操作是,用噬菌体 Eryne 感染含有大肠杆菌 K-12 MG1655 ΔRM wbbL (+) 宿主的 LB 软琼脂 ... 平均覆盖度为 165×。研究人员利用 Pharokka 1.7.3 版本和 Phold 0.2.0 版本,采用标准设置对噬菌体 Eryne 的基因组进行注释,之后将其线性化,5′端位于大终止酶基因的起始密码 ...
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