研究人员采用了多种关键技术方法。其中，单分子荧光原位杂交（smFISH）技术用于在单细胞水平可视化 Fos ecRNA 和 mRNA 转录本，以探究它们的分布和相关性；通过构建表达重组人蛋白的质粒，表达并纯化了 DNMT3A 和 DNMT3L 等蛋白；运用计算建模预测 Fos - 1 ecRNA 与 ...

In situ validation – whether through smFISH for RNA detection or, even more critically, protein-based approaches such as immunostaining – would offer a more comprehensive and robust picture of these ...

在研究的结论和讨论部分，此次研究意义重大。它是迄今为止对 1 型糖尿病患者胰腺肠道病毒感染指标进行的最大规模、最全面的分析。研究结果表明，肠道病毒感染与胰岛自身免疫相关，无论是在临床前期还是诊断后，都可能对 β 细胞的丢失产生直接或间接的影响。这一发现为 1 型糖尿病的发病机制提供了新的见解，支持了肠道病毒感染在 1 型糖尿病发展过程中的重要作用。

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. An evolutionarily conserved micropeptide, ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Mood disorders (MDs) are caused by an ...

The need for definitive answers probably explains our natural tendency to seek simplicity. The reductionist “bulk” approach, in which a mean behavior is attributed to a heterogeneous cell population, ...

The Nikon BioImaging Lab USA provides preclinical contract research services for microscope-based imaging, analysis and assay development to biotech, pharma, and larger research communities.

1. 胃癌前病变的分子特征：研究通过整合基因表达、空间转录组学、单细胞RNA测序（scRNA-seq）和smFISH技术，鉴定出26个基因的高风险特征，这些基因 ...

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel Center for Environmental Genomics, University of Washington, Seattle, United States Center for Anatomy & Cell Biology, ...