在成功开发出用于病毒重建的自辅助病毒启动方法后，作者随后建立了一个合成基因组学的基因组组装过程来组装非洲猪瘟病毒基因组，这类似于作者先前报道的用于人类疱疹病毒的方法。作者通过酵母中的转化相关重组（TAR）技术，从 12 个重叠的野生型或修饰片段（或 ...

你知道吗？非洲猪瘟，这种被称为养猪业头号杀手的病毒，终于迎来了重大突破！近日，美国克雷格·文特尔研究所的研究团队在《Science Advances》杂志上发表了重磅研究成果，他们成功开发出一种基于合成基因组学的非洲猪瘟病毒工程平台，这将为疫苗研发和治疗方法的开发打开新的大门。 非洲猪瘟是一种致命性病毒性疾病，对全球养猪业造成了巨大的经济损失。它主要分布在非洲、欧洲、亚洲以及加勒比海的伊斯帕尼奥拉 ...

Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular and molecular basis governing microglia maintenance ...

Not revised: This Reviewed Preprint includes the authors’ original preprint (without revision), an eLife assessment, public reviews, and a provisional response from the authors. The article entitled ...

5-HT下行易化系统通过5-HT2C受体对脊髓PKCγ神经元去抑制并导致神经病理性痛觉超敏The 5-HT Descending Facilitation System Contributes to the Disinhibition of ...

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The protein samples were separated by SDS-PAGE and then transferred to polyvinylidene difluoride (PVDF) membranes (Roche, Switzerland). PVDF membranes were blocked with 5% skimmed milk diluted in TBST ...

The need for definitive answers probably explains our natural tendency to seek simplicity. The reductionist “bulk” approach, in which a mean behavior is attributed to a heterogeneous cell population, ...

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