在研究方法上，研究人员采用了多种关键技术。利用免疫共沉淀（CoIP）技术筛选与 Vpx rcm 相互作用的细胞蛋白；运用 RNA 干扰（RNAi）技术沉默 KRT72 的表达，以此评估其对 HIV-1 感染的影响；通过细胞实验，包括对多种细胞系和原代细胞的研究，深入探究 KRT72 和 ...

近日，首都医科大学基础医学院口腔健康北京实验室王松灵院士团队在《Science Bulletin》上发表题为“Nitrate ameliorates myelin loss and cognitive impairment in Alzheimer's disease through upregulation of neuronal sialin and subsequent inhibition ...

蛋白质相互作用研究：采用质谱（MS）技术以 HAVCR1 为诱饵，鉴定其相互作用蛋白。通过免疫共沉淀（coIP）、酶联免疫吸附测定（ELISA）等实验验证蛋白质之间的相互作用。 研究结果 CRISPR 筛选鉴定关键基因：经 CRISPR 筛选，获得 48 个 ZIKV 感染人滋养层细胞所需的 ...

The protein samples were separated by SDS-PAGE and then transferred to polyvinylidene difluoride (PVDF) membranes (Roche, Switzerland). PVDF membranes were blocked with 5% skimmed milk diluted in TBST ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Influenza A virus (IAV) Matrix 2 protein (M2) ...