All samples were acquired and analyzed by flow cytometry. Measurement of Metabolic Parameters by Flow Cytometry PBMCs were activated overnight by anti-CD3/CD28 beads (Gibco). Cells were stained with ...

Background: Anti-CD19 chimeric antigen receptor T (CAR-T) cell therapy has proven effective for treating relapsed or refractory acute B cell leukemia. However, challenges such as cytokine release ...

Revised: This Reviewed Preprint has been revised by the authors in response to the previous round of peer review; the eLife assessment and the public reviews have been updated where necessary by the ...

研究结果如下： NAT10 在 T 细胞激活过程中上调：研究发现，在 T 细胞激活过程中，NAT10 的表达会显著增加。通过体外实验，用抗 CD3/CD28 刺激小鼠脾 T N 细胞，发现 NAT10 蛋白表达在 6、24 和 48 小时显著上升；在体内实验中，利用急性淋巴细胞性脉络丛脑膜炎病毒 ...

2024年，阿尔茨海默病（AD）治疗领域迎来“抗体疗法元年”。继2023年礼来（Eli Lilly）的Donanemab和卫材（Eisai）/渤健（Biogen）的 ...

细胞培养技术也是研究的关键环节，他们分别对人骨髓间充质干细胞（MSCs）、人脐血造血干细胞（HSCs）进行培养，并通过特定方法制备人抗 CD3/CD28 激活的 T 淋巴细胞。同时，利用流式细胞术（FACS）对细胞进行分析，准确检测细胞表面标记和细胞内成分 ...