10). I became interested in this first ubiquitin conjugate, UbH2A. Back in Russia, I had begun to develop a method for high-resolution analysis of nucleosomes. These DNA–protein complexes were ...

A 'crossover' loop restricts access to the active site and might prevent binding of large, fully folded ubiquitin conjugates (see the figure, part e, left). DUBs are also responsible for the ...

To prepare site-specific protein-ubiquitin and protein-SUMO conjugates we have devised a chemical approach based on Cu(I)-catalyzed click chemistry (1-4). This bioorthogonal cycloaddition between an ...