“We are getting good at predicting how to do it.” Once the antisense siRNA strand goes into the RISC, there are some attributes of the siRNA sequence that correlate with efficiency. Dharmacon ...

Eubacterial Argonaute Enzymes. Ago proteins constitute a key component of the RNA-induced silencing complex (RISC), contributing to both the architectural and catalytic functionalities associated with ...

Investigators are seizing the opportunity to use siRNA technology to silence their favourite genes. Recently, the successful use of siRNA in whole mice was reported. It has recently been shown ...

Viroid replication generates dsRNA intermediates, which are processed by Dicer into 21- to 25-nucleotide siRNAs. These siRNAs are then incorporated into siRNA – ribonuclease complexes (RISC). If the ...

Gene silencing techniques Description of mechanism Ref. siRNA The siRNA associates with RISC leading to unwinding of the dsRNA, sense strand of which is cleaved by Argonaut2 protein. The antisense ...