A deeper look into the apparent self-defense mechanisms of these bacteriophage-resistant bacteria revealed their secret weapon: a group of enzymes called restriction endonucleases, or restriction ...

Various types of endonucleases – enzymes that can cut DNA – were already known before CRISPR-Cas9. The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology.

General sub-cloning or PCR cloning into the multiple cloning sites with feasible restriction site(s) to use and only involves the 20 common restriction endonucleases listed below. (List of the 20 ...