为解决向日葵茎溃疡病菌（Diaporthe helianthi）检测难题，研究人员开发 RPA/CRISPR-Cas12a 检测体系，灵敏度高且适用于现场检测。 向日葵，作为世界主要油料作物之一，不仅在食用油生产方面占据重要地位，还广泛应用于医药、化妆品领域，甚至非油用向日葵还具有 ...

【导读】人类疾病的多效应和基因相互作用网络的复杂性要求基因敲入小鼠能够进行多重基因扰乱。 2025年3月20日，耶鲁大学 ...

Pines studied a detection strategy that combined RPA and CRISPR technology. RPA amplifies the DNA, and when CRISPR-Cas12a binds to the target DNA, the Cas12a begins to indiscriminately chop up ...

The platform utilizes a combined approach involving recombinant polymerase amplification (RPA), the CRISPR/Cas12a system, and an immunochromatographic test strip (ICS). It specifically targets the ...

coli. Selecting the appropriate Cas12a among the many orthologs for a given target sequence remains difficult. Here, Peng Chen et al. evaluate the activity and specificity of Cas12a variants and ...

The new tool, which is called CRISPR-Cas12a, can help researchers simultaneously assess the impact of multiple genetic changes involved in variety of immune system responses, the researchers say. “We ...