RNase H结合越慢，运动中的停顿时间就越长，这导致整体处理时间变慢。 通过增加RNase H的浓度，速度明显提高，暂停时间从70秒减少到0.2秒左右。

Bacterial mRNAs begin with a triphosphate on the first transcribed nucleotide, but RNase E, the endonuclease long thought to initiate mRNA decay in Escherichia coli, only works well on RNA with a ...