To prepare the RNA standard, synthesize it in vitro by transcription of the appropriate strand of a recombinant plasmid containing the DNA sequences of interest and a bacteriophage promoter.

The articles in this series discuss different aspects of the biogenesis of mRNAs and other RNA species (including microRNAs, rRNAs, tRNAs, small RNAs and other non-coding RNAs), the regulation of ...

The resulting RNA molecule, typically mRNA, carries the genetic message from the nucleus to the cytoplasm for protein synthesis. Nucleic acids are the foundation of genetic engineering techniques, ...

Recombinant proteins may also have compromised structural integrity due to the presence of protease in the apoplast. To evade ...