Mix one volume of this 2× calcium phosphate–DNA solution with an equal volume of 2× HEPES-buffered saline (HBS) at 15–25 °C. Tap the side of the tube to mix and incubate the solution at 15 ...

1| Wash thirty 10-cm plates of the appropriate cells (a total of ∼6 × 10 7 cells) in phosphate-buffered saline. Scrape each plate of cells into 1 ml of ice-cold EBC lysis buffer (50 mM Tris (pH ...