研究人员主要采用了动物实验、细胞培养和多种检测技术来开展研究。在动物实验方面，构建了小鼠闭合性头部损伤模型模拟重复性轻度创伤性脑损伤（r-mTBI）；细胞培养则包括对小鼠脑血管周细胞和人脑血管内皮细胞的培养；同时运用 ELISA、免疫荧光、Western blotting 等检测技术，对相关蛋白表达、细胞增殖和细胞因子分泌等进行测定。

as illustrated by platelet-derived growth factor (PDGF)-BB stimulation of different porcine aortic endothelial (PAE) cells (Fig. 2). Wild-type PAE cells do not express human PDGFR, PAE-α cells ...

The study further demonstrates that OTUB1 is necessary for platelet-derived growth factor-BB (PDGF-BB)-induced proliferation, phenotype switching, and migration of HASMCs. RNA sequencing and mass ...