Conditions of the PCR amplification are as follows: 94°C (5 min), then 30 cycles at 94°C (30 s) / 56°C (45 s) / 72°C (45 s), followed by 8 cycles 94°C (30 s) / 53°C (45 s) / 72°C (45 s ...

Following synthesis and at the end of the first cycle, each double-stranded DNA molecule consists of one new and one old DNA strand. PCR then continues with additional cycles that repeat the ...

the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time RT–PCR. Fast and accurate, real-time RT–PCR ...

Credit: Courtesy of The Smithsonian Institution The "aha" moment and initial experiments in 1983 through which Kary Mullis developed the idea of polymerase chain reaction (PCR) are a well trodden ...