While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

The emergence of imaging schemes capable of overcoming Abbe's diffraction barrier is revolutionizing optical microscopy. In 1873, the German physicist Ernst Abbe realized that the resolution of ...

Experimental setup for the multimodal nonlinear optical microscopy of multiphoton excitation fluorescence (MPEF) and second harmonic generation (SHG) imaging. A femtosecond pulse from a tunable ...

While fuorescence has become the dominant mode in biological microscopy nowadays for its high molecular contrast ... These phase shifted images can then be combined, using phase retrieval equations ...

Researchers at the University of Adelaide, as part of an international team, have developed an approach that makes advanced microscopy possible through an optical fiber thinner than a human hair.