The high specificity exhibited by I-CreI derives from its recognition of a long, 22-base-pair DNA target sequence and the integrated structure ... nuclease is generated through an iterative ...

This led to the identification of AcrVIB1, the first anti-CRISPR protein targeting the Cas13b nuclease ... at HIRI have teamed up to understand the structure of the inhibition mechanism better.

The ongoing major projects in the laboratory focus on the structural biology of CRISPR-Cas surveillance complexes and their suppression by anti-CRISPR proteins (in collaboration with Luciano ...

The complex adopts a more compact structure when bound to double-stranded DNA (dsDNA), with the target DNA strand making a pronounced U-turn and interacting with the HNH nuclease domain.