Place the tip of the pipette into the V-shaped groove of the hemocytometer and allow the cell suspension to flow into the chamber of the hemocytometer by capillary action until the chamber is filled.

and compared 350 iQ200 body fluid cell counts with manual hemacytometer cell counts. Within-run imprecision, expressed as coefficient of variation (CV), ranged from 2.6% to 5.9% for RBC counts ...