Accordingly, the total loss of DNA ligase function is lethal. DNA ligases catalyze the joining of a 5’-phosphate-terminated strand to a 3’-hydroxyl-terminated strand. Ligation depends on magnesium and ...

Ubiquitylation requires the subsequent actions of three enzymes: an activating E1, a conjugating E2 and an E3 ligase. Hoeller et al. now show that monoubiquitylation of ubiquitin-binding domain ...