coli. Our findings demonstrate that fusion of GFP to amino acidic tags also affects GFP folding in eukaryotic cells. Remarkably, specific protein tags can not only reduce, but also dramatically ...

The pET11a-link-NGFP vector incorporates an N-terminal hexahistidine tag. Because GFP reassembly is effectively irreversible, and unassembled GFP-fragment fusions are typically insoluble ...