Hence, it is unclear what determines the orientation of PIC assembly and, thus, the direction of transcription. To provide the structural basis for the polarity of transcription, we determined the ...

Many eukaryotic genes have a conserved TATA box located 25-35 base pairs before the transcription start site of a gene. The TATA box is able to define the direction of transcription and also ...

the single-stranded DNA is exposed and ready for transcription. At this point, RNA polymerase begins moving down the DNA template strand in the 3' to 5' direction, and as it does so, it strings ...