The assay uses antibodies that selectively bind cGAMP in the presence of excess ATP and GTP. When the fluorescent ... and minimizes compound interference. cGAS titration at 1 hour and 24 hours ...

cGAS was shown to be a member of the nucleotidyltransferase family and to be capable of generating cGAMP in vitro from GTP and ATP in the presence of dsDNA. cGAMP in turn bound to and activated the ...

The first is cyclic GMP-AMP (cGAMP), a molecule that switches on STING when foreign DNA lurks in cells. The second is cGAS, the enzyme that enables the cells of mammals — including humans — to ...

However, Chen wanted to figure out the enzyme that made cGAMP. Through another series of biochemical fractionation and quantitative mass spectrometry, Chen narrowed his search to identify the enzyme ...

The new assay measures the enzymatic activity of cyclic GMP-AMP synthase (cGAS) by directly detecting the product cGAMP, in a simple mix-and-read format. Based on the proven Transcreener platform, ...