Membranes not used immediately in hybridization reactions should be thoroughly dried, wrapped loosely in aluminum foil or blotting paper and stored ... Cloning: A Laboratory Manual (eds.

Use alkaline transfer buffer (0.4 M NaOH with 1 M NaCl) for charged nylon membranes. 9| Place a piece of thick blotting paper on a sheet of Plexiglas or a glass plate to form a support that is ...