This protocol describes the first stages of Southern blotting ... 3| Separate the fragments of DNA by electrophoresis through an agarose gel (for example, a 0.7% gel cast in 0.5× TBE or 1× ...

The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

It is analogous to Southern blotting, which is used to analyze DNA. This protocol describes the transfer of RNA from agarose gels to nylon membranes and the fixation of the RNA to the membrane 1,2,3.

However, this confirmatory nature can also be used later in studies, for example confirming the success of a gene-editing technique such as CRISPR. This In Focus will examine the capabilities of ...