Avoid fusions to the GFP fragments at the site of protein-protein interaction and ... constructs into a DE3 host (we use electrocompetent BL21). When a single pair of constructs is to be checked ...

The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia ... Each plasmid was separately transformed into the BL21(DE3) expression host.

coli BL21 Star (DE3) on LB plates with 50 µg/mL kanamycin ... To accelerate enzyme engineering, researchers used cell-free ...