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RR%2bin%2bCHO%2fh(P)RR-10His%2bcells.%2b(A)%2bThe%2bS1P%2bmRNA%2blevels%2bin%2bCHO%2fh(P)RR-10His%2bcells%2btreated%2bwith%2beither%2bS1P%2bsiRNA%2b(siS1P)%2bor%2bnegative%2bcontrol%2bsiRNA%2b(siNC)%2bwere%2bmeasured%2bby%2bqRT-PCR%2b(n%2b%253D%2b3%253B%2b**P%2b%3c%2bversus%2bsiNC).%2b(B)%2bWhole%2bcell%2blysates%2b(10%2b%c2%b5g%2bof%2btotal%2bprotein)%2band%2bculture%2bsupernatants%2b(one-quarter%2bof%2bthe%2bcollected%2bamount)%2bwere%2banalysed%2bby%2bSDS-PAGE%252C%2bfollowed%2bby%2bimmunoblotting%2busing%2banti-(P)RR%2bantibodies.%2bNS%2bindicates%2bnon-specific%2bbands.%2bGraphs%2bin%2bB%2brepresent%2bsemiquantitative%2bmeasurements%2bof%2bthe%2bfull-length%2b(P)RR%2b%5bFL-(P)RR-10His%5d%2band%2bs(P)RR%2bbands%2busing%2bImageJ%2bsoftware%2b(NIH%252C%2bBethesda%252C%2bMD%252C%2bUSA%253B%2bn%2b%253D%2b3%253B%2b***P%2b%3c%2bversus%2bsiNC%253B%2b****P%2b%3c%2bversus%2bsiNC).%2bRepresentative%2bresults%2bof%2bthree%2bsimilar%2bindependent%2bexperiments%2bare%2bshown..jpg&cdnurl=https%3a%2f%2fth.bing.com%2fth%2fid%2fR.a163f8082ab56e8257985fced4731600%3frik%3dX93UuAv6Pe5oYw%26pid%3dImgRaw%26r%3d0&exph=768&expw=1024&q=SR+P1&simid=608009229449839889&FORM=IRPRST&ck=CC52F12F77DDAF4F81DFC382953BA9A5&selectedIndex=30&itb=0)
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RR%2bin%2bCHO%2fh(P)RR-10His%2bcells.%2bWhole%2bcell%2blysates%2b(15%2b%c2%b5g%2bof%2btotal%2bprotein)%2band%2bculture%2bsupernatants%2b(one-tenth%2bof%2bthe%2bcollected%2bamount)%2bprepared%2bfrom%2bCHO%2fh(P)RR-10His%2bcells%2bthat%2bhad%2bbeen%2btransfected%2bwith%2beither%2bthe%2bexpression%2bplasmid%2bcoding%2bfor%2bhuman%2bS1P%2bor%2bthe%2bempty%2bplasmid%2b(Vector)%2bwere%2banalysed%2bby%2bSDS-PAGE%252C%2bfollowed%2bby%2bimmunoblotting%2busing%2beither%2banti-S1P%2b(A)%2bor%2banti-(P)RR%2bantibodies%2b(B).%2bRepresentative%2bblots%2bof%2bthree%2bindependent%2bexperiments%2beach%2bwith%2bthree%2bindependent%2btreatments%2bare%2bshown.%2bNS%2bindicates%2bnon-specific%2bbands.%2bGraphs%2brepresent%2bsemiquantitative%2bmeasurements%2bof%2bthe%2bfull-length%2b(P)RR%2b%5bFL-(P)RR-10His%5d%2band%2bs(P)RR%2bbands%2bof%2bthree%2bindependent%2bexperiments%2busing%2bImageJ%2bsoftware%2b(NIH%253B%2bn%2b%253D%2b3%2fgroup%253B%2b*P%2b%3c%2b0.05%2bversus%2bVector)..jpg&cdnurl=https%3a%2f%2fth.bing.com%2fth%2fid%2fR.21e289d5bc76c1722837836c50779dac%3frik%3d4HKqxmDSaQY42Q%26pid%3dImgRaw%26r%3d0&exph=768&expw=1024&q=SR+P1&simid=608010754206033855&FORM=IRPRST&ck=FA68E14CB0BF10BE60C92C667BE34FF1&selectedIndex=29&itb=0){kind=link}